Paper-based DPPH Assay for Antioxidant Activity Analysis
نویسندگان
چکیده
منابع مشابه
Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins
The 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assay is well established for the in vitro determination of antioxidant activity in food and biological extracts. The standard DPPH assay uses methanol or ethanol as solvents, or buffered alcoholic solutions in a ratio of 40%/60% (buffer/alcohol, v/v) to keep the hydrophobic hydrazyl radical and phenolic test compounds soluble while offering sufficient ...
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Snezana Agatonovic-Kustrin1*, David Mortona1 and Pauzi A. Yusof2 1School of Pharmacy and Applied Science, La Trobe Institute of Molecular Sciences, La Trobe University, Bendigo, Australia 2Physiology Department, Medical School, Universiti Teknologi Mara, Selangor, Malaysia *Corresponding author: Agatonovic KS. School of Pharmacy and Applied Science, La Trobe Institute of Molecular Sciences, La ...
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Guava fruit extracts were analyzed for antioxidant activity measured in methanol extract (AOAM), antioxidant activity measured in dichloromethane extract (AOAD), ascorbic acid, total phenolics, and total carotenoids contents. The ABTS, DPPH, and FRAP assays were used for determining both AOAM and AOAD, whereas the ORAC was used for determining only AOAM. Averaged AOAM [mM Trolox equivalent (TE)...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2018
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.18p014